Immobilization of Aspergillus niger Pectinase on Polyacrylonitrile Copolymer Membrane
نویسندگان
چکیده
منابع مشابه
Production kinetics and characterization of pectinase enzyme from Aspergillus niger
Food grade pectinases from Aspergillus niger are predominantly used as processing aids in industries. In the present investigation, efforts towards standardization for pilot scale production of pectinase enzyme from A. niger were made. The crude enzyme was studied for its kinetics to optimize substrate concentration and processing parameters on the basis of polygalacturonic activity. Km (Michae...
متن کاملAffinity precipitation of Aspergillus niger pectinase by microwave-treated alginate.
Affinity precipitation is a simple, single plate separation process in which the complex of a smart macroaffinity ligand with the target protein (from a crude broth) can be selectively precipitated by application of a suitable stimulus. Alginate is a copolymer of guluronic acid and mannuronic acid residues and precipitates with Ca(2+) ions. It was found to bind to pectinase present in a commerc...
متن کاملImmobilization of polymethylgalacturonase producing Aspergillus niger on Luffa sponge material.
The vegetable sponge of Luffa cylindrica was studied as a matrix for the immobilization of Aspergillus niger 26, producer of polymethylgalacturonase (PMG). Entrapped spores could grow and multiply within the lattice of the sponge. The influence of loofa sponge inoculum content, initial spore inoculum content, and duration of the growth cycle on the enzyme activity and mycelium growth was studie...
متن کاملDegradation of pectic polysaccharides in various fruits by pectinase derived from Aspergillus niger
Aspergillus spp. are widely spread plant pathogens. In several kinds of fruit, especially in apple and peach, aspergillus rot is one of the postharvest desease in fruit, but the infection mechanism is not known. The degradation of cell walls by pathogens is significant in primary infection and afterinfection, and plays an important role in the advancement of mycelium into the host plant and the...
متن کاملISOLATION, PRODUCTION AND CHARACTERIZATION OF EXTRACELLULAR PECTINASE FROM Aspergillus niger K3
A pectinase producing fungus was isolated from compost soil. Morphological characteristics, microscopic observations and molecular characterization based on internal transcribed spacer (ITS) sequences led to its identification as Aspergillus niger (GenBank Accession Number: JX556221) and it was designated as Aspergillus niger strain K3. In the present study, the culture condition of the media w...
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ژورنال
عنوان ژورنال: Trends in Applied Sciences Research
سال: 2007
ISSN: 1819-3579
DOI: 10.3923/tasr.2007.419.425